A positive displacement pipette is a syringe-type device that uses a piston to aspirate liquid. Unlike other pipette tips, positive displacement pipettes do not have an air cushion between the piston and liquid. This feature helps increase the accuracy of volume measurements and dispensing highly viscous samples. This type of pipette uses disposable Capillary Pistons.
The Positive Displacement tip is a great tool for accurate and contamination-free pipetting of viscous solutions or liquids with high vapor pressure. The integrated piston prevents contamination and ensures accurate pipetting of highly viscous solutions. These pipettes are highly recommended for critical PCR protocols. They also work with standard air displacement pipettors and can be a more economical alternative. However, positive displacement pipette tips are the preferred choice for high-quality sample delivery.
A positive displacement pipette tip should be held at a 20-degree angle when using it. You should never lay your pipette on a bench. Always store it on a stand to avoid any liquid residue buildup. This prevents the piston from misalignment and lubricant accumulation. If you do not remove the tip after pipetting, the liquid may evaporate and enter the pipette body.
Choosing the correct type of tip is crucial to the accuracy of your pipetting. If you work with hazardous liquids, such as radioactive solutions, you should choose a positive displacement pipette. The air cushion inside the tips allows corrosive samples to evaporate and can also damage the pipette's piston, seal, and shaft. The end result of this is a poor quality sample. These tips should not be used in human experiments.
A positive displacement pipette is a handy tool for biomedical researchers. This tool can help optimize sample preparation for qPCR, HPLC, and pharmaceutical formulations. They can also aliquot volatile liquids without causing leakage, which prevents contamination of the sample by vapors. The Microman positive displacement pipette is a great option for a laboratory that uses volatile liquids.
Another advantage of using positive displacement pipette tip is that there is no air cushion, which reduces the accuracy of measurements. This means that even volatile, hazardous, and viscous liquid samples can be pipetted with ease. The first step in cleaning a positive displacement pipette is setting the volume you require. Then, you should press the plunger button and move the piston to the first stop position, allowing the liquid to pass through the capillary.
If you're working in a laboratory, you've probably seen an Eppendorf pipette, which is a common lab tool. Pipettes have colored tips, each representing different dosages, and a plunger button that indicates the range of the pipette. Care should be taken when handling the pipettes, however, as improper use can lead to permanent damage. It's important to always store pipettes in an upright position, to avoid damaging them and their components.
The Eppendorf Research plus features 50 years of innovation in liquid handling to make it one of the most ergonomic pipettes available today. Its design ensures low tip attachment and ejection forces and it's easy to autoclave without disassembling the device. The Eppendorf Research plus comes in single and two-pipetting styles. You can also find multichannel pipettes for larger samples. This comprehensive range of pipettes can be used for a variety of applications, from biochemical analysis to pharmaceutical research.